Abstract: This study evaluated the regulatory effects of plant growth regulators on in vitro propagation of distinct organ types in Adiantum nelumboides X. C. Zhang, using rhizome tips, sterile gametophytes, and sporophytes as explants. Results showed that kinetin (KT) and indole-3-butyric acid (IBA) promoted normal shoot tip growth, while 6-benzylaminopurine (6-BA) most effectively promoted shoot meristem proliferation. Application of 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus formation from growing points. In gametophyte cultures, 0.5 mg/L 6-BA maximized the induction and proliferation of globular green bodies (GGB), whereas 1.0 mg/L zeatin (ZT) most effectively promoted GGB differentiation. In sporophyte regeneration, 0.5 mg/L naphthaleneacetic acid (NAA) yielded the highest rooting rate and enhanced both GGB-derived seedling differentiation and sporophyte growth in liquid MS medium. These findings clarify the organ-specific hormonal responses in A. nelumboides and offer a foundational framework for optimizing large-scale ex situ propagation protocols for this endangered species.