不同念珠藻的提取物对酪氨酸酶活性的抑制作用

范羽仪; 胡征宇; 梅洪

不同念珠藻的提取物对酪氨酸酶活性的抑制作用

Tyrosinase Inhibitory Activities of Methanolic Extracts from Different Nostoc Species

摘要

摘要: 研究了Nostoc sp.FACHB87、Nostoc paludosum FACHB89等10株念珠藻的甲醇提取物对酪氨酸酶的抑制作用。结果表明,随着提取物浓度的升高,对酪氨酸酶的抑制作用也增强。其中Nostocsp.FACHB892的甲醇提取物对酪氨酸酶抑制作用最强,在33.3μg/mL的最高测试浓度下,抑制率可达到60.54%;Nostoc sp.FACHB95、Nostoc sp.FACHB106、N.punctiforme FACHB252、N.calcicola FACHB389和N.parmiloidesFACHB392的甲醇提取物,在33.3μg/mL的最高测试浓度下,抑制率分别为41.55%、37.58%、46.94%、52.12%和47.95%。Nostoc sp.FACHB892的甲醇提取物对酪氨酸酶的抑制作用表现为可逆抑制,抑制类型为非竞争型抑制。

Abstract: The inhibitory effects on tyrosinase of the methanolic extracts from 10 Nostoc strains were investigated.The results show that the inhibitory action for the above 6 strains of Nostoc enhanced with the increase of concentration of the methanolic extracts.The methanolic extracts of Nostoc sp.FACHB892 has great inhibitory capacity on tyrosinase,of which the inhibitory rate is 60.54% at concentration of 33.3 μg/mL,while the methanolic extracts of other 5 strains(Nostoc sp.FACHB95,Nostoc sp.FACHB106,N.punctiforme FACHB252,N.calcicola FACHB389 and N.parmiloides FACHB392) exhibitted weaker inhibitory effects.The inhibitory rates are 41.55%,37.58%,46.94%,52.12% and 47.95% respectively at the same concentration.The inhibitory mechanism of the methanolic extracts of Nostoc sp.FACHB892 on tyrosinase is reversible and noncompetitive indicated by the enzymatic kinetic analysis.

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