Abstract: Metabonomics is a new discipline that has emerged after proteomics and genomics, and is mainly applied to study biological samples and small molecule metabolites in organs. Currently, it is widely used in biomedicine, food science, agricultural zoology, botany, and other fields. This paper summarizes the application of metabolomics technology in the regulation of plant secondary metabolism as well as the research status of metabolomics in popular fields of plant research in the past five years to provide a theoretical basis for the further development of metabolomics research.