Abstract: The whole genome sequence of Dictamnus dasycarpus Turcz. chloroplasts was obtained by high-throughput sequencing, and its structural features and phylogenetic relationships were analyzed by bioinformatics. Results showed that the chloroplast genome had a typical circular tetrameric structure with a total length of 157 139 bp, a large and small single-copy region of 84 478 bp and 18 587 bp, respectively, a pair of inverted repeat sequences of 27 037 bp, and a total GC content of 38.5%. In total, 132 genes were annotated, including 87 protein-coding genes (PCGs), eight ribosomal RNA (rRNA) genes, and 37 transfer RNA (tRNA) genes. The frequency of relative synonymous codon use (RSCU) was calculated, showing that the most encoded codon was leucine (Leu/L) and the least encoded codon was cysteine (Cys/C), with 32 codons with RSCU values greater than 1. The D. dasycarpus chloroplast genome contained a total of 61 simple repetitive sequences. Compared with eight species of Rutaceae, the chloroplast genome structure was generally consistent between Dictamnus and other genera, with no rearrangements or inversions. Phylogenetic analysis showed that D. dasycarpus was most closely related to Orixa japonica Thunb.