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王峰, 卢永恩, 李汉霞. 几种白菜类蔬菜卡那霉素抗性的研究[J]. 植物科学学报, 2006, 24(4): 377-380.
引用本文: 王峰, 卢永恩, 李汉霞. 几种白菜类蔬菜卡那霉素抗性的研究[J]. 植物科学学报, 2006, 24(4): 377-380.
WANG Feng, LU Yong-En, LI Han-Xia. Kanamycin Resistance of Several Vegetable Brassicas[J]. Plant Science Journal, 2006, 24(4): 377-380.
Citation: WANG Feng, LU Yong-En, LI Han-Xia. Kanamycin Resistance of Several Vegetable Brassicas[J]. Plant Science Journal, 2006, 24(4): 377-380.

几种白菜类蔬菜卡那霉素抗性的研究

Kanamycin Resistance of Several Vegetable Brassicas

  • 摘要: 标记基因的利用是筛选和鉴定基因转化的细胞、组织和转基因植株的有效方法。针对目前遗传转化中常用的卡那霉素(Km)抗性基因,对几种白菜类蔬菜的卡那霉素抗性作了较系统的探索,发现5.0 mg/L Km可完全抑制大白菜、小白菜及菜心子叶的再生;幼苗Km抗性测验表明各品种均表现出随着Km浓度的提高,子叶黄化率升高、根茎变短、鲜重减轻的趋势。白菜类蔬菜的卡那霉素抗性检测为遗传转化和阳性后代筛选奠定了基础。

     

    Abstract: The use of marker gene is an efficient method to select and identify transgenic cell,tissues and plantlets.In the light of kanamycin resistance gene widely used in plant genetic transformation,a(syste-)(matic) investigation that contributes to genetic transformation on resistance of several vegetable brassicas is made in this experiment.The tests showed that 5 mg/L kanamycin could completely inhibit regeneration of cotyledon of vegetable brassicas.Kanamycin resistance assay of seedlings suggested the tendency that(cotyledon) yellow rates increase,root and stem length decrease,and fresh weigh decrease with the level of kanamycin improved.This provided a base for genetic transformation of vegetable brassicas and screening of their offspring.

     

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