Detection of the effects of exogenous signal molecules on ATP levels in Arabidopsis thaliana(L.)Heynh. using fluorescence resonance energy transfer
Da Xiao-Wei1, Sun Min1, Wang Xin1, Pang Hai-Long1,2, Jia Ling-Yun1,2, Feng Han-Qing1,2
1. College of Life Science, Northwest Normal University, Lanzhou 730370, China;
2. New Rural Development Research Institute, Northwest Normal University, Lanzhou 730070, China
We used an adenosine triphosphate (ATP) fluorescence protein-sensor (Ateam1.03-nD/nA) based on fluorescence resonance energy transfer (FRET) to analyze the effects of extracellular ATP, Ca2+, H2O2, and NO on ATP levels in the cytoplasm and chloroplasts of Arabidopsis thaliana seedlings. Results showed that ATP levels were higher in the cytoplasm than in the chloroplasts. Under treatment with different concentrations of the signal molecules, the FRET ratio of plastid-localized Ateam 1.03-nD/nA fluctuated non-significantly between 1.2 and 1.8. The FRET ratio of cytosol-localized Ateam1.03-nD/nA fluctuated non-significantly between 2.2 and 3.0. These results indicate that both cytoplasmic ATP and chloroplast ATP maintained relatively stable levels upon stimulation by signal molecules at different intensities.
达晓伟, 孙敏, 王馨, 庞海龙, 贾凌云, 冯汉青. 利用荧光共振能量转移检测外源信号分子对拟南芥ATP水平的影响[J]. 植物科学学报, 2022, 40(3): 390-397.
Da Xiao-Wei, Sun Min, Wang Xin, Pang Hai-Long, Jia Ling-Yun, Feng Han-Qing. Detection of the effects of exogenous signal molecules on ATP levels in Arabidopsis thaliana(L.)Heynh. using fluorescence resonance energy transfer. Plant Science Journal, 2022, 40(3): 390-397.
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2022, Vol. 40 
