水盐条件变化对三江藨草叶绿素荧光特性的影响

doi:10.11913/PSJ.2095-0837.2021.60654

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摘要

以吉林莫莫格湿地常见植物三江藨草(Scirpus nipponicus Makino)为材料，设置6个盐浓度梯度和6个水位梯度进行室内控制实验，对三江藨草叶片的叶绿素含量和荧光参数进行测定，以探讨该植物在光合过程中对水盐条件变化的响应机制。结果显示：随着水位升高，叶绿素荧光参数均呈先增加后降低的趋势；在低水位范围(-10 ~ 20 cm)处理下，F_v/F_o、F_v/F_m、q_N值较高，而Yield、ETR和q_P在高水位环境(40 ~ 80 cm)中较高。随着盐度的升高，三江藨草叶绿素含量和荧光参数均呈先增加后降低的趋势，即当盐浓度升高至3000 mg/L时，各指标含量下降。研究结果表明，三江藨草适宜生长在40 ~ 80 cm水位较高的环境中，具有一定的耐盐性，且高水位能够缓解盐胁迫对光合结构造成的伤害，提高其对盐环境的耐受性。

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	马琼芳
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	张超凡

关键词 ：湿地植物, 水位, 盐度, 叶绿素含量, 叶绿素荧光

Abstract：

To evaluate the effects of hydrology and salinity on chlorophyll fluorescence parameters of Scirpus nipponicus Makino in Momoge National Nature Reserve, we conducted a controlled experiment with six different levels of salinity (300, 1000, 2000, 3000, 4000, and 5000 mg/L) and six different water depths (-10, 0, 20, 40, 60, and 80 cm). A SPAD-502 plus portable chlorophyll content tester and Junior-PAM modulated chlorophyll fluorometer were used to measure chlorophyll content and fluorescence parameters, respectively. Results showed that as the water level increased, chlorophyll content increased and chlorophyll fluorescence parameters (i.e., potential activity of PSⅡ, maximal quantum yield of PSⅡ, actual quantum yield of PSⅡ in light, photosynthetic electron transfer rate, photochemical quenching, and non-photochemical quenching) increased at first and then decreased. Furthermore, F_v/F_o, F_v/F_m, and q_N were higher in the low-water range (-10 - 20 cm), while yield, ETR, and q_P were higher in high-water range (40 - 80 cm). The chlorophyll content and fluorescence parameters of S.nipponicus initially increased with increasing salinity but decreased when the salt concentration reached 3000 mg/L. These results indicate that S. nipponicus is suitable for growing in high water level environments (40 - 80 cm) and exhibits a certain tolerance for salinity, with the higher water levels alleviating damage caused by salt stress to allow photosynthesis and improve tolerance to high salt environments.

Key words： Wetland plant Water level Salinity Chlorophyll content Chlorophyll fluorescence

收稿日期: 2021-02-20

ZTFLH:

Q945.11

基金资助:

国家重点研发计划（2016YFC0500407）；吉林省林业厅科技项目（2015-004）；吉林省公益性科研院所科研项目（GY-2020-06）；林业科技创新平台运行补助项目（2020132032）

通讯作者: 张超凡,E-mail:455762808@qq.com E-mail: 455762808@qq.com

作者简介: 马琼芳(1984-),女,博士,副研究员,研究方向为湿地生态学(E-mail:youzi841128@qq.com)

引用本文:

马琼芳, 燕红, 孔维尧, 陈玲, 李伟东, 邹畅林, 张超凡. 水盐条件变化对三江藨草叶绿素荧光特性的影响[J]. 植物科学学报, 2021, 39(6): 654-662. MA Qiong-fang, YAN Hong, KONG Wei-yao, CHEN Ling, LI Wei-dong, ZOU Chang-lin, ZHANG Chao-fan. Effects of hydrology and salinity on chlorophyll fluorescence parameters of Scirpus nipponicus Makino. Plant Science Journal, 2021, 39(6): 654-662.

链接本文:

http://www.plantscience.cn/CN/10.11913/PSJ.2095-0837.2021.60654 或 http://www.plantscience.cn/CN/Y2021/V39/I6/654

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