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李雪丽, 耿甜甜, 汪颖, 钱婷, 张玉洁, 赵凡, 孙坤, 张辉. 基于RNA-seq技术对乌头属铁棒锤中自交不亲和S基因的挖掘与分析[J]. 植物科学学报, 2021, 39(2): 172-182. DOI: 10.11913/PSJ.2095-0837.2021.20172
引用本文: 李雪丽, 耿甜甜, 汪颖, 钱婷, 张玉洁, 赵凡, 孙坤, 张辉. 基于RNA-seq技术对乌头属铁棒锤中自交不亲和S基因的挖掘与分析[J]. 植物科学学报, 2021, 39(2): 172-182. DOI: 10.11913/PSJ.2095-0837.2021.20172
Li Xue-Li, Geng Tian-Tian, Wang Ying, Qian Ting, Zhang Yu-Jie, Zhao Fan, Sun Kun, Zhang Hui. Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N. Busch based on RNA-seq[J]. Plant Science Journal, 2021, 39(2): 172-182. DOI: 10.11913/PSJ.2095-0837.2021.20172
Citation: Li Xue-Li, Geng Tian-Tian, Wang Ying, Qian Ting, Zhang Yu-Jie, Zhao Fan, Sun Kun, Zhang Hui. Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N. Busch based on RNA-seq[J]. Plant Science Journal, 2021, 39(2): 172-182. DOI: 10.11913/PSJ.2095-0837.2021.20172

基于RNA-seq技术对乌头属铁棒锤中自交不亲和S基因的挖掘与分析

Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N. Busch based on RNA-seq

  • 摘要: 以毛茛科乌头属铁棒锤(Aconitum pendulum N.Busch)2个品系‘蓝花铁棒锤’(‘WSYB1’)和‘黄花铁棒锤’(‘WSYY1’)为材料,对其进行转录组测序(RNA-seq),采用生物信息学方法鉴定其中可能存在的花柱S基因(self-incompatibility gene)和花粉S基因,并对它们的序列特征进行分析。结果显示,转录组中共鉴定出2个在雌蕊中特异或高表达的花柱S基因(ApSRNase)和2个在雄蕊中特异表达的花粉S基因(ApSLF)。与耧斗菜(Aquilegia coerulea James)相似,铁棒锤中也存在S-RNaseS locus ribonucleases)和SLFS locus F-box)控制的S-RNase类的自交不亲和系统,而不存在sS(stigma S-determinant)和pS(pollen S-determinant)控制的罂粟科类型的自交不亲和系统。

     

    Abstract: Two Aconitum pendulum N. Busch cultivars (‘WSYB1’ and ‘WSYY1’) were used as materials for transcriptome sequencing (RNA-seq) using bioinformatics to identify possible style and pollen S genes and analyze their sequence characteristics. Results identified two style S genes (ApSRNase) specifically or highly expressed in the style and two pollen S genes (ApSLF) specifically expressed in pollen. Similar to the sequenced plant Aquilegia coerulea James, this study showed that there was a S-RNase-based self-incompatibility system controlled by S-RNase and SLF in A. pendulum, but no Papaveraceae-type self-incompatibility system controlled by sS and pS.

     

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