一种改良的大豆线粒体DNA提取方法

An improved method to extract Glycine max mitochondrial DNA

摘要: 以大豆（Glycine max（L.） Merrill）黄化苗为材料，通过优化提取线粒体DNA （mtDNA）时差速离心过程中的离心力和离心时间，以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度，结合高盐法去除蛋白质，改良大豆mtDNA的提取方法。结果表明，该方法提取的mtDNA浓度和纯度较高，无叶绿体和核基因组DNA的污染，可用于后续大豆线粒体基因组的相关研究。

Abstract: In this study, yellowing Glycine max(L.) Merrill seedlings were used to extract mitochondrial DNA(mtDNA) by optimizing centrifugal force and time during differential centrifugation. Furthermore, establishing different sucrose density gradients and lysate concentrations during purification, combined with high salt removal protein, improved the extraction method of soybean mtDNA. Results showed that the concentration and purity of mtDNA extracted by this method were high, with no chloroplast or nuclear genomic DNA contamination, the mtDNA could be used for subsequent research on soybean mitochondrial genome.