Abstract:
In this study, the promoter region of the
DGAT2 gene was isolated and characterized from oil palm (
Elaeis guineensis Jacq.). Based on genomic DNA sequence analysis, a 1035 bp promoter region was amplified by PCR. Several functional elements, such as TATA-box and CAAT-box, were identified from the obtained sequence by alignment in the database. To verify the tissue specificity of the
GUS gene in different tissues, we constructed the
DGAT2 promoter expression vector that contained the
GUS gene and transformed the vector into
Arabidopsis thaliana via the floral dip method. GUS staining of transgenic
A.thaliana indicated that the
DGAT2 promoter can drive gene expression without significant tissue specificity but showed obvious discrepancies in different organs by real-time PCR.