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祖奎玲, 董树斌, 李建霞, 徐申健, 赵良成. 南蛇藤假种皮形成过程中萜类合成基因的表达分析[J]. 植物科学学报, 2017, 35(2): 276-282. DOI: 10.11913/PSJ.2095-0837.2017.20276
引用本文: 祖奎玲, 董树斌, 李建霞, 徐申健, 赵良成. 南蛇藤假种皮形成过程中萜类合成基因的表达分析[J]. 植物科学学报, 2017, 35(2): 276-282. DOI: 10.11913/PSJ.2095-0837.2017.20276
Zu Kui-Ling, Dong Shu-Bin, Li Jian-Xia, Xu Shen-Jian, Zhao Liang-Cheng. Differentially expressed genes analysis of terpenoid biosynthesis related to aril development in Celastrus orbiculatus Thunb.[J]. Plant Science Journal, 2017, 35(2): 276-282. DOI: 10.11913/PSJ.2095-0837.2017.20276
Citation: Zu Kui-Ling, Dong Shu-Bin, Li Jian-Xia, Xu Shen-Jian, Zhao Liang-Cheng. Differentially expressed genes analysis of terpenoid biosynthesis related to aril development in Celastrus orbiculatus Thunb.[J]. Plant Science Journal, 2017, 35(2): 276-282. DOI: 10.11913/PSJ.2095-0837.2017.20276

南蛇藤假种皮形成过程中萜类合成基因的表达分析

Differentially expressed genes analysis of terpenoid biosynthesis related to aril development in Celastrus orbiculatus Thunb.

  • 摘要: 为探究南蛇藤(Celastrus orbiculatus Thunb.)假种皮发育过程中萜类物质合成相关基因的表达差异,本文选取3个不同发育时期(盛花期、落花期和幼果期)的南蛇藤雌花为实验材料,构建3个cDNA文库,通过Illumina HiSeqTM 2000平台进行转录组测序,共获得87600个unigene序列,平均长度为453bp,N50/N90比值为4.64。通过3个不同发育阶段基因差异表达的比较及功能富集分析,鉴定出16个萜类合成相关基因,其中8个基因的编码蛋白参与调控7个萜类合成代谢位点。还确定了与萜类合成相关的7个转录因子家族的成员数量以及相对表达量最高的基因。

     

    Abstract: To research differentially expressed genes (DEGs) related to terpenoid biosynthesis in the developmental process of aril in Celastrus orbiculatus Thunb., three cDNA libraries from three stages (full blooming, flower senescence, and young fruit) were established and then sequenced by Illumina HiSeqTM 2000. RNA-Seq was assembled into 87 600 unigenes with a mean length of 453 bp and N50/N90 of 4.46. By differential expression analysis of pairwise comparisons and functional enrichment, 21 DEGs were confirmed to have highly differential expression related to terpenoid biosynthesis. Among them, eight genes regulated the metabolism of seven terpenoid synthetases. We also identified seven transcription factor families and high expression genes associated with terpenoid synthesis.

     

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