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文书生, 成仿云, 钟原, 王新, 李刘泽木, 黄弄璋. ‘正午’牡丹微繁殖体系的建立[J]. 植物科学学报, 2016, 34(1): 143-150. DOI: 10.11913/PSJ.2095-0837.2016.10143
引用本文: 文书生, 成仿云, 钟原, 王新, 李刘泽木, 黄弄璋. ‘正午’牡丹微繁殖体系的建立[J]. 植物科学学报, 2016, 34(1): 143-150. DOI: 10.11913/PSJ.2095-0837.2016.10143
WEN Shu-Sheng, CHENG Fang-Yun, ZHONG Yuan, WANG Xin, LI Liu-Zemu, HUANG Nong-Zhang. Protocol for the Micropropagation of Tree Peony (Paeonia × lemoinei ‘High Noon’)[J]. Plant Science Journal, 2016, 34(1): 143-150. DOI: 10.11913/PSJ.2095-0837.2016.10143
Citation: WEN Shu-Sheng, CHENG Fang-Yun, ZHONG Yuan, WANG Xin, LI Liu-Zemu, HUANG Nong-Zhang. Protocol for the Micropropagation of Tree Peony (Paeonia × lemoinei ‘High Noon’)[J]. Plant Science Journal, 2016, 34(1): 143-150. DOI: 10.11913/PSJ.2095-0837.2016.10143

‘正午’牡丹微繁殖体系的建立

Protocol for the Micropropagation of Tree Peony (Paeonia × lemoinei ‘High Noon’)

  • 摘要: 本研究以‘正午’牡丹腋芽为外植体,建立了高效的牡丹微繁殖体系.腋芽的初始培养基为WPM+ 0.5 mg/L BA+ 0.2 mg/L GA3,培养50 d 后,一个丛生芽平均可切分为13个繁殖体用于增殖培养;增殖培养基为WPM1668 mg/L Ca(NO3)2·4 H2O+ 0.5 mg/L BA+ 0.2 mg/L GA3,以35 d为一个继代培养周期,增殖率为3.0,共继代培养7次;生根培养时,先将无根苗在复壮培养基1/2 MS (296 mg/L CaCl2)+ 0.5 g/L 活性炭上培养20 d,再转入根诱导培养基1/2 MS (296 mg/L CaCl2)+ 1.0 mg/L腐胺+ 1.0 mg/L IBA培养30 d,最后转入根形成培养基1/2 MS (296 mg/L CaCl2)+ 4.0 g/L 活性炭培养20 d,其生根率达77.2%;驯化与移栽基质为珍珠岩:蛭石:草炭土 = 1:1:1,组培苗移栽成活率高达92.1%.这表明以‘正午’牡丹腋芽建立的微繁殖体系具备规模化商业生产的价值.

     

    Abstract: An efficient micropropagation protocol was developed for Paeonia × lemoinei ‘High Noon’ using axillary buds as explants. During initiation, 13 propagules were obtained after 50 d of culture with WPM + 0.5 mg/L BA + 0.2 mg/L GA3. During proliferation, three shoots were obtained after 35 d of culture with WPM1668 mg/L Ca(NO3)2·4 H2O + 0.5 mg/L BA + 0.2 mg/L GA3, and seven subcultures were carried out. For optimal rooting, the shoots were cultured with 1/2 MS (296 mg/L CaCl2) + 0.5 g/L activated charcoal for 20 d, then 1/2 MS (296 mg/L CaCl2) + 1.0 mg/L putrescine + 1.0 mg/L IBA for 30 d for root induction, and finally 1/2 MS (296 mg/L CaCl2) + 4.0 g/L activated charcoal for 20 d for root development. The rooting percentage of the shoots was 77.2%. During acclimatization, the rooted plantlets were transferred to pots containing a mix of vermiculite/peat/perlite (1:1:1 V/V/V) substrate, and the survival rate was 92.1%. The micropropagation protocol for Paeonia × lemoinei ‘High Noon’ established in this study is valuable for commercial use.

     

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