高效液相色谱法同时测定荷叶中6种黄酮类成分

doi:10.11913/PSJ.2095-0837.2015.60861

摘要
图/表
参考文献(0)
相关文章 (15)

全文: PDF (734 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

为了探索同时测定荷叶茶及饮片中6种黄酮类成分(芦丁、金丝桃苷、紫云英苷、槲皮素、山奈素和异鼠李素)含量的方法,本研究以8种不同的荷叶样本为材料,采用高效液相色谱分析法对6种黄酮类成分进行了同时测定。样品经过前处理,以0.5%甲酸-水(A)和0.1%甲酸-乙腈(B)作为流动相进行梯度洗脱,流速为1 mL/min,柱温为25℃,进样量为20 μL,经Agilent TC-C18(2) (150 mm × 4.6 mm, 5 μm)色谱柱分离,于360 nm 波长处检测,结果显示,芦丁、金丝桃苷、紫云英苷、槲皮素、山奈酚、异鼠李素6种黄酮类成分分别在1.6 ~ 160、1.8 ~ 180、2.16 ~ 216、1.4 ~ 140、2.12 ~ 212、1.6 ~ 160 μg/mL浓度范围内有良好的线性关系(R² > 0.9992),其检测稳定性、重复性、日内精密度、日间精密度以及加样回收率的RSD均小于2%。进一步用该方法对不同来源的8个荷叶样本进行检测,结果显示荷叶样本中6种黄酮类成分含量以槲皮素最高,并且以样本G荷叶茶(购自G公司,批号为130802)的槲皮素含量最高。本研究建立的同时测定6种荷叶黄酮类成分含量的方法快速、准确,可为荷叶有效成分的检测和质量控制提供技术支撑。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	梁佳文
	刘艾洁
	马冰馨
	王有为

关键词 ：高效液相色谱, 同时测定, 荷叶, 黄酮

Abstract：

To simultaneously determine the contents of six flavonoids (rutin, hyperoside, astragalin, quercetin, kaempferol and isorhamnetin) in lotus leaves and related tea products, eight lotus leaf samples from different companies were detected by HPLC. The prepared samples were separated by Agilent TC-C18(2) (150 mm × 4.6 mm, 5 μm) at 25℃ with a step linear gradient using 0.5% formic acid-water (A) and 0.1% formic acid-acetonitrile (B) at a flow rate of 1.0 mL/min. The detection wavelength was 360 nm. Results showed that the concentration ranges of rutin, hyperoside, astragalin, quercetin, kaempferide and isorhamnetin were 1.6-160, 1.8-180, 2.16-216, 1.4-140, 2.12-212 and 1.6-160 μg/mL, respectively. Between these ranges, the linearity of the six flavonoids were well-presented (R² > 0.9992) in a certain concentration range. The RSD values for the stability, repeatability, intra-day and inter-day precisions and recovery rates were all less than 2%. Eight lotus leaf samples from different companies were detected by the above method. The content of quercetin was the highest among the six flavonoids, and was highest in Batch No. 130802 from Company G. The method used in this study accurately detected the contents of six flavonoids in lotus leaves and tea products, and provided an effective way in which to determine the functional components and quality of lotus leaf products.

Key words： HPLC Simultaneous determination Lotus leave Flavonoids

收稿日期: 2015-06-04

ZTFLH:

Q946.8

基金资助:

国家“十二五”科技支撑计划项目(2012BAI29B03);湖北省自然科学基金重点项目(2010CBB02301)。

作者简介: 梁佳文(1988-),女,硕士研究生,从事中药质量标准研究(E-mail:127236288@qq.com)。

引用本文:

梁佳文, 刘艾洁, 马冰馨, 王有为. 高效液相色谱法同时测定荷叶中6种黄酮类成分[J]. 植物科学学报, 2015, 33(6): 861-866. LIANG Jia-Wen, LIU Ai-Jie, MA Bing-Xin, WANG You-Wei. Simultaneous Determination of Six Flavonoid Compounds in Lotus Leaves by High Performance Liquid Chromatography. Plant Science Journal, 2015, 33(6): 861-866.

链接本文:

http://www.whzwxyj.cn/CN/10.11913/PSJ.2095-0837.2015.60861 或 http://www.whzwxyj.cn/CN/Y2015/V33/I6/861

[1] 国家药典委员会.中华人民共和国药典: 一部(2010年版)[M]. 北京: 中国医药科技出版社, 2010.
[2] Huang B, Ban XQ, He JS, Tong J, Tian J, Wang YW. Hepatoprotective and antioxidant activity of ethanolic extracts of edible lotus (Nelumbo nuci-fera Gaertn.) leaves[J]. Food Chem, 2010, 120(3): 873-878.
[3] 张兰英,董长颖. 荷叶生物碱的提取及体外抑菌和凝血活性研究[J]. 特产研究, 2012(2): 17-19.
[4] Liu S, Li D, Huang B, Chen YX, Lu XC, Wang YW. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves[J]. J Ethnopharmacol, 2013, 149(1): 263-269.
[5] 林秀英. 一种由普洱茶茶熟茶、晒青荷叶、晒青干姜混合的泡腾饮品: CN201210242737.5. 2012-10-17.
[6] 梁伟. 鲜竹荷叶茶及其生产方法:CN201210582122.7. 2013-04-24.
[7] 黄友谊,肖平,张博炜,徐莉萍,许雷,谭雪,倪超. 一种荷叶金花菌茶及其制备方法:CN201210162197.X. 2013-01-09.
[8] 张松波. 一种荷叶苦瓜茶及其制作方法:CN2012- 10176969.5. 2012-09-19.
[9] 刘军波,邹礼根,赵芸. 荷叶固体饮料配方及风味研究[J]. 饮料工业, 2013, 16(12): 13-15.
[10] 何晓曦,孔令提,张泽生,叶林虎,刘新民,曾建国,常琪. 不同产地和不同采收期荷叶中生物碱的含量变化[J]. 天然产物研究与开发, 2013, 25(5): 652-655, 661.
[11] Chen S, Zheng Y, Fang JB, Liu YL, Li SH. Flavonoids in lotus (Nelumbo) leaves evaluated by HPLC-MSⁿ at the germplasm level[J]. Food Res Int, 2013, 54(1): 796-803.
[12] 朱燕超, 陶文沂. 酶法制备荷叶黄酮苷元的研究[J].天然产物研究与开发, 2008, 20(5): 773-777.
[13] 廖思红, 陈建军, 王瑛.偏斜淫羊藿化学成分研究[J].植物科学学报, 2013, 31(4): 385-390.
[14] 刘贤青, 张红艳. HPLC-Q-TOF/MS分析脐橙果实中的类黄酮[J]. 植物科学学报, 2014, 32(6): 638-644.
[15] 唐超,张学兰,徐鑫.荷叶炮制金丝桃苷含量变化研究[J].山东中医药大学学报, 2011, 35(1): 78.
[16] 袁丽春,刘斌,石任兵.HPLC法测定不同市售荷叶药材中金丝桃苷和异槲皮苷的含量[J]. 药物分析杂志, 2010, 30(1): 41.
[17] 王新财,陈晓平. 反相高效液相色谱法测定荷叶中异槲皮苷含量[J]. 医药导报, 2012, 11(31): 1505-1507.
[18] 李慧芬,张学兰,崔伟亮,刘洋. HPLC 同时测定不同产地荷叶饮片中4种黄酮成分[J]. 中国实验方剂学杂志, 2014, 20(2): 55-58.
[19] Chen S, Fang L, Xi H, Guan L, Fang J, Liu Y, Wu B, Li S. Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus (Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry[J]. Anal Chim Acta, 2012, 724(8): 127-135.
[20] Chen S, Wu BH, Fang JB, Lu YL, Zhang HH, Fang LC, Guan L, Li SH. Analysis of flavonoids from lotus (Nelumbo nucifera) leaves using high performance liquid chromatography/photodiode array detector tandem electrospray ionization mass spectrometry and an extraction method optimized by orthogonal design[J]. J Chromatogr A, 2012, 1277(2): 145-153.
[21] Yang RZ, Wei XL, Gao FF, Wang LS, Zhang HJ, Xu YJ, Li CH, Zhang JJ, Zhang J. Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry[J]. J Chromatogr A, 2009, 1216: 106-112.
[22] Deng J, Chen S, Yin XJ, Wang K, Liu YL, Li SH, Yang PF. Systematic qualitative and quantitative assessment of anthocyanins, flavones and flavonols in the petals of 108 lotus (Nelumbo nucifera) cultivars[J]. Food Chem, 2013, 139(1-4): 307-312.
[23] Wang DL, Tang W, Yang GM. Anti-inflammatory, antioxidant and cytotoxic activities of flavonoids from Oxytropis falcata Bunge[J].Chin J Nat Med, 2010,8(6): 461-465.
[24] Zhang XR, Liu XR, Liu YC. Cardiovascular protective effects of soy isoflavone[J]. Chin J Clinical Rehab, 2005, 9 (7): 241-243.
[25] 王其超,张行言. 中国荷花品种图志[M]. 北京:中国林业出版社,2004: 21.
[26] 高丽,操得群,刘芳,邓芳玉,刘贝. 高效液相色谱法测定荷叶中槲皮素的含量[J]. 中国酿造, 2012, 31(6): 175-177.
[27] 李慧芬,崔伟亮,张学兰.荷叶炮制前后4种黄酮类成分含量变化[J]. 时珍国医国药, 2014, 25(1): 89-91.