高级检索+
曹建国, 陈雪菲, 谢颖华, 张敏, 王全喜. 水蕨(Ceratopteris thalictroides)查尔酮合成酶基因的克隆和表达[J]. 植物科学学报, 2015, 33(4): 489-498. DOI: 10.11913/PSJ.2095-0837.2015.40489
引用本文: 曹建国, 陈雪菲, 谢颖华, 张敏, 王全喜. 水蕨(Ceratopteris thalictroides)查尔酮合成酶基因的克隆和表达[J]. 植物科学学报, 2015, 33(4): 489-498. DOI: 10.11913/PSJ.2095-0837.2015.40489
CAO Jian-Guo, CHEN Xue-Fei, XIE Ying-Hua, ZHANG Min, WANG Quan-Xi. Isolation and Expression Profiling of Gene Encoding Chalcone Synthase in Ceratopteris thalictroides[J]. Plant Science Journal, 2015, 33(4): 489-498. DOI: 10.11913/PSJ.2095-0837.2015.40489
Citation: CAO Jian-Guo, CHEN Xue-Fei, XIE Ying-Hua, ZHANG Min, WANG Quan-Xi. Isolation and Expression Profiling of Gene Encoding Chalcone Synthase in Ceratopteris thalictroides[J]. Plant Science Journal, 2015, 33(4): 489-498. DOI: 10.11913/PSJ.2095-0837.2015.40489

水蕨(Ceratopteris thalictroides)查尔酮合成酶基因的克隆和表达

Isolation and Expression Profiling of Gene Encoding Chalcone Synthase in Ceratopteris thalictroides

  • 摘要: 查尔酮合酶(chalcone synthase, CHS)是植物类黄酮化合物合成的关键酶,有关蕨类植物CHS基因的序列及功能信息尚不完善。本研究采用快速扩增cDNA末端(RACE)技术克隆获得了模式蕨类植物——水蕨(Ceratopteris thalictroides)CtCHS基因(GenBank登录号:JX027616.1),其cDNA序列全长为1616 bp,具有3个外显子和2个内含子,开放阅读框(ORF)为1215 bp,编码404个氨基酸。进化树分析表明,CtCHS与问荆(Equisetum arvense)、松叶蕨(Psilotum nudum)和3种薄囊蕨的查尔酮合成酶基因聚为一枝,说明这些蕨类植物亲缘关系较近且为单系起源。通过构建原核表达体系成功获得CtCHS蛋白的多克隆抗体并用于免疫印迹分析,结果表明CtCHS基因的表达明显受紫外光(UV)诱导。CtCHS基因的克隆与表达分析为进一步研究水蕨类黄酮化合物的合成及其调控机制提供了依据。

     

    Abstract: Chalcone synthase (CHS) is a key enzyme in the synthesis of plant flavonoids. However, information on CHS genes in ferns is still unclear. In this study, rapid amplification of cDNA ends (RACE) was used to isolate the full-length sequence of the CHS gene from model fern Ceratopteris thalictroides (CtCHS, GenBank accession number: JX027616.1). Sequence analysis showed that the full length of the CtCHS gene was 1616 bp, with three exons and two introns. Its ORF region was 1215 bp, encoding 404 amino acids. Phylogenetic analysis indicated that CtCHS was clustered with the other ferns, including Equisetum arvense, Psilotum nudum and three leptosporangiate ferns, which reflected the monophyletic feature of the ferns according to Smith's system. Western blot analysis showed that the expression of this gene was significantly affected by ultraviolet (UV) treatment. In this study, the full-length sequence of CtCHS was cloned and the function of the CtCHS protein was studied, thus providing molecular information for further studies on the effect of CtCHS on flavonoids production.

     

/

返回文章
返回