Cloning and Functional Analysis of COR27 from Vitis vinifera
FAN Gao-Tao1,2, SUN Xiao-Ming2, REN Xiao-Die3, LI Shao-Hua2, XIN Hai-Ping2, WANG Wan-Jun1
1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;
2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
3. School of Environmental Studies, China University of Geosciences, Wuhan 430074, China
Low winter temperature is one of the main factors that affect the development of grapes and the wine industry in China. Understanding the signal transduction pathway during cold stress will help in the breeding of high cold-resistance cultivars. Based on our previous transcriptome analysis, a gene that showed increased expression pattern in Vitis vinifera L. ‘Muscat Hamburg’ during cold treatment was identified and named VvCOR27 according to homological analysis. The whole length of VvCOR27 cDNA was 1082 bp, which contained a 909 bp open reading frame (ORF) and encoded 302 amino acids. Homological analysis of COR27s from thirteen species showed that they contained three COR27-specific conservative domains. Real time RT-PCR indicated that the transcript abundance of VvCOR27 was highly increased at 24 h after cold treatment. Four motifs, including EE, EEL, G-box and ABREL, were found in the promoter regions (from published Vitis vinifera genome sequences) of VvCOR27 but at less quantity than that in the promoter regions of AtCOR27. This may be why AtCOR27 showed more timely responses to cold treatment than did VvCOR27. Phenotypic analysis of three overexpression lines under cold treatment indicated that VvCOR27 was involved in responses to cold stress and enhanced cold tolerance in plants.
[1] 贺普超. 葡萄学[M]. 北京:中国农业出版社, 2001.
[2] 孔庆山. 中国葡萄志[M]. 北京:中国农业科技出版社, 2004.
[3] 宋润刚, 路文鹏, 王军, 沈育杰, 林兴桂, 葛玉香, 李晓红, 孙克娟. 山葡萄品种选育回顾与展望[J]. 北方园艺, 1999, 129: 36-38.
[4] 林兴桂. 我国酿酒葡萄抗寒育种的回顾与展望[J]. 果树学报, 2007, 24(1): 89-93.
[5] 刘军, 王小伟, 魏钦平, 鲁韧强, 高照全. 世界葡萄抗寒育种的成就与展望[J]. 果树学报, 2004, 21(5): 461-466.
[6] 贺普超, 牛立新. 我国葡萄属野生种抗寒性的研究[J]. 园艺学报, 1989, 11(1): 81-88.
[7] Jin WM, Dong J, Hu YL, Lin ZP, Xu XF, Han ZH. Improved cold-resistant performance in transgenic grape (Vitis vinifera L.) overexpressing cold-inducible transcription factors AtDREB1b[J]. Hortscience, 2009, 44(1): 35-39.
[8] Tillett LR, Wheatley MD, Tattersall EAR, Schlauch KA, Cramer GR, Cushman JC. The Vitis vinifera C-repeat binding protein 4 (VvCBF4) transcriptional factor enhances freezing tolerance in wine grape[J]. Plant Biotechnology J, 2012, 10(1): 105-124.
[9] Zhang JZ, Creelman RA, Zhu JK. From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops[J]. Plant Physiol, 2004, 135(2): 615-621.
[10] Zhou MQ, Shen C, Wu LH, Tang KX, Lin J. CBF-dependent signaling pathway: a key responder to low temperature stress in plants[J]. Crit Rev Biotechnol, 2011, 31(2): 186-92.
[11] Xiao H, Siddiqua M, Braybrook S, Nassuth A. Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid[J]. Plant Cell Environ, 2006, 29(7): 1410-1421.
[12] Xiao H, Tattersall EA, Siddiqua MK, Cramer GR, Nassuth A. CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia[J]. Plant Cell Environ, 2008, 31(1): 1-10.
[13] Li JT, Wang LN, Zhu W, Wang N, Xin HP, Li SH. Characterization of two VvICE1 genes isolated from ‘Muscat Hamburg’ grapevine and their effect on the tolerance to abiotic stresses[J]. Sci Hortic, 2014a, 165(22): 266-273.
[14] Li JT, Wang N, Wang LN, Xin HP, Li SH. Molecular cloning and characterization of the HOS1 gene from ‘Muscat Hamburg’ grapevine[J]. J Am Soc Hortic Sci, 2014b, 139(1): 54-62.
[15] Liu LY, Li H. Research progress in amur grape, Vitis amurensis Rupr.[J]. Can J Plant Sci, 2013, 93(4): 565-575.
[16] Dong C, Tao JM, Zhang M, Qin Y, Yu ZY, Wang BL, Cai BH, Zhang Z. Isolation and expression characterization of CBF2 in Vitis amurensis with stress[J]. Agr Sci, 2013a, 4(9): 466-472.
[17] Dong C, Zhang M, Yu ZY, Ren JP, Qin Y, Wang BL, Xiao LZ, Zhang Z, Tao JM. Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress[J]. Agr Sci, 2013b, 4(5): 224-229.
[18] Dong C, Zhang Z, Qin Y, Ren JP, Huang JF, Wang BL, Lu HL, Cai BH, Tao JM. VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance[J]. Acta Physiol Plant, 2013c, 35(10): 2975-2984.
[19] Dong C, Zhang Z, Ren JP, Qin Y, Huang JF, Wang Y, Cai BH, Wang BL, Tao JM. Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco[J]. Plant Physiol Bioch, 2013d, 71(2013): 212-217.
[20] Li JT, Wang N, Xin HP, Li SH. Overexpression of VaCBF4, a transcription factor from Vitis amurensis, improves cold tolerance accompanying increased resistance to drought and salinity in Arabidopsis[J]. Plant Mol Biol Rep, 2013, 31(6): 1518-1528.
[21] Mikkelsen MD, Thomashow MF. A role for circa-dian evening elements in cold-regulated gene expression in Arabidopsis[J]. Plant J, 2009, 60(2): 328-339.
[22] Kilian J, Whitehead D, Horak J, Wanke D, Weinl S, Batistic O, D'Angelo C, Bornberg-Bauer E, Kudla J, Harter K. The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses[J]. Plant J, 2007, 50(2): 347-363.
[23] Xin HP, Zhu W, Wang LN, Xiang Y, Fang LC, Li JT, Sun XM, Wang N, Londo J, Li SH. Genome wide transcriptional profile analysis of Vitis amurensis and V. vinifera in response to cold stress[J]. PLoS One, 2013, 8(3): e58740.
[24] Xu W, Li R, Zhang N, Ma F, Jiao Y, Wang Z. Transcriptome profiling of Vitis amurensis, an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress[J]. Plant Mol Biol, 2014, 86(4-5): 527-541.
2015, Vol. 33 
