高级检索+
吴锦程, 吴毕莎, 黄审剑, 陈祠贤, 颜亮, 许宏斌, 林建琼. 枇杷幼果PLD和LOX对低温胁迫的响应[J]. 植物科学学报, 2015, 33(2): 203-209. DOI: 10.11913/PSJ.2095-0837.2015.20203
引用本文: 吴锦程, 吴毕莎, 黄审剑, 陈祠贤, 颜亮, 许宏斌, 林建琼. 枇杷幼果PLD和LOX对低温胁迫的响应[J]. 植物科学学报, 2015, 33(2): 203-209. DOI: 10.11913/PSJ.2095-0837.2015.20203
WU Jin-Cheng, WU Bi-Sha, HUANG Shen-Jian, CHEN Ci-Xian, YAN Liang, XU Hong-Bin, LIN Jian-Qiong. Phospholipase D and Lipoxygenase of Young Loquat Fruits in Response to Low Temperature Stress[J]. Plant Science Journal, 2015, 33(2): 203-209. DOI: 10.11913/PSJ.2095-0837.2015.20203
Citation: WU Jin-Cheng, WU Bi-Sha, HUANG Shen-Jian, CHEN Ci-Xian, YAN Liang, XU Hong-Bin, LIN Jian-Qiong. Phospholipase D and Lipoxygenase of Young Loquat Fruits in Response to Low Temperature Stress[J]. Plant Science Journal, 2015, 33(2): 203-209. DOI: 10.11913/PSJ.2095-0837.2015.20203

枇杷幼果PLD和LOX对低温胁迫的响应

Phospholipase D and Lipoxygenase of Young Loquat Fruits in Response to Low Temperature Stress

  • 摘要: 以3年生枇杷品种 ‘早钟6号’(Eriobotrya japonica ‘Zaozhong No.6’)容器嫁接苗为试材,于0℃、-1℃、-3℃人工气候室内进行低温胁迫处理,探讨枇杷幼果细胞膜磷脂及相关酶对低温胁迫的响应机制。结果显示,在不同温度胁迫过程中,枇杷幼果磷脂酶D (PLD, EC 3.1.4.4)和脂氧合酶(LOX, EC 1.13.11.12)活性均呈上升趋势;质膜磷脂酰胆碱(PC)和磷脂酰肌醇(PI)含量因逐渐被降解而呈下降趋势,磷脂酸(PA)含量出现积累、增加,而膜结合Ca2+含量有不同程度的降低。随处理时间的延长和处理温度的降低,枇杷幼果细胞PLD和LOX活性增幅加大,从而加速了膜PC和PI的降解和PA的积累。低温胁迫过程中幼果细胞膜PC含量的降幅大于PI,膜结合Ca2+含量的变化与PLD和LOX活性变化呈负相关。低温胁迫下枇杷幼果细胞膜结合Ca2+含量的减少诱导了膜脂降解酶PLD和LOX活性的提高,并导致膜结构稳定性下降,加剧了低温胁迫对膜脂的降解和脂质过氧化伤害,其中尤以-3℃胁迫处理4~6 h对幼果细胞质膜的伤害最严重。表明低温胁迫下Ca2+·CaM信使系统可能参与枇杷幼果细胞膜PLD和LOX活性的调控。

     

    Abstract: Container grafted seedlings of three-year-old loquat (Eriobotrya japonica ‘Zaozhong No.6’) were treated at 0℃, -1℃ and -3℃ in phytotron to determine the mechanism of responses of membrane phospholipid and related enzymes in young loquat fruits under low temperature stress. Results showed that the activities of phospholipase D (PLD, EC 3.1.4.4) and lipoxygenase (LOX, EC 1.13.11.12) increased in young loquat fruits under different low temperature stress. The contents of phosphatidylcholine (PC) and phosphatidylinositol (PI) decreased due to gradual degradation. Phosphatidic acid (PA) accumulations increased, and the membrane-associated Ca2+ contents were reduced to different degrees. With increasing stress time and decreasing stress temperature, the activities of PLD and LOX increased, resulting in accelerated PC, PI degradations and PA accumulation. The decrease in PC content was greater than that of PI content in the membranes of young loquat fruits under low temperature stress. There was a negative correlation between PLD, LOX activities and membrane-associated Ca2+ contents. The increase in PLD and LOX activities was caused by the decrease in membrane-associated Ca2+ contents. Meanwhile, the decrease in membrane-associated Ca2+ contents resulted in stable decreases in membrane structure, which aggravated membrane damage due to lipid degradation and peroxidation. The damage to the cell membranes of young fruits was most serious under low temperature stress at -3℃ for 4-6 h. The signal system of Ca2+·CaM may be involved in regulating PLD and LOX activities of young loquat fruits under low temperature stress.

     

/

返回文章
返回