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程芳婷, 李忠虎, 刘春艳, 原超, 李雪童, 刘占林. 地黄属植物的DNA条形码研究[J]. 植物科学学报, 2015, 33(1): 25-32. DOI: 10.11913/PSJ.2095-0837.2015.10025
引用本文: 程芳婷, 李忠虎, 刘春艳, 原超, 李雪童, 刘占林. 地黄属植物的DNA条形码研究[J]. 植物科学学报, 2015, 33(1): 25-32. DOI: 10.11913/PSJ.2095-0837.2015.10025
CHENG Fang-Ting, LI Zhong-Hu, LIU Chun-Yan, YUAN Chao, LI Xue-Tong, LIU Zhan-Lin. DNA Barcoding of the Genus Rehmannia (Scrophulariaceae)[J]. Plant Science Journal, 2015, 33(1): 25-32. DOI: 10.11913/PSJ.2095-0837.2015.10025
Citation: CHENG Fang-Ting, LI Zhong-Hu, LIU Chun-Yan, YUAN Chao, LI Xue-Tong, LIU Zhan-Lin. DNA Barcoding of the Genus Rehmannia (Scrophulariaceae)[J]. Plant Science Journal, 2015, 33(1): 25-32. DOI: 10.11913/PSJ.2095-0837.2015.10025

地黄属植物的DNA条形码研究

DNA Barcoding of the Genus Rehmannia (Scrophulariaceae)

  • 摘要: 地黄属(Rehmannia)为玄参科(Scrophulariaceae)药用植物,广泛分布于中国中东部及北部地区。由于地黄属植物经历了快速成种,导致其属内物种间形态性状差异较小,运用传统的形态学分类方法已难以准确地鉴定物种,近年来迅速发展起来的DNA条形码技术为快速、准确地鉴别物种提供了新思路。本研究选用3个叶绿体DNA非编码区片段(trnL-trnF、trnM-trnV和trnS-trnG)及核基因ITS片段,运用PWG-distance和Tree-Building两种方法对地黄属5个物种75个个体进行了DNA条形码分析。结果表明:单个叶绿体DNA片段或核基因ITS片段对地黄属物种的鉴别率较低(0%~20%),组合的叶绿体DNA片段分辨能力虽然高于单个DNA片段,但并不能将地黄属5个物种完全区分开;trnS-trnG+ITS片段组合的分辨率可达100%,能够将地黄属5个物种准确区分,与所有叶绿体DNA片段和核基因ITS片段组合(trnL-trnF+trnM-trnV+trnS-trnG+ITS)的辨别率相同,因此推荐trnS-trnG+ITS作为地黄属植物的标准条形码。此外,利用DNA条形码鉴别物种时,可采用叶绿体DNA片段和核DNA片段组合的方法来提高物种鉴定的成功率。

     

    Abstract: Rehmannia, a genus of Scrophulariaceae, is widely distributed in central, eastern and northern China. However, it is relatively difficult to distinguish species by traditional morphological methods due to rapid speciation of the genus. Recently developed DNA barcoding provides a new approach to identify species quickly and accurately. In this study, three candidate DNA noncoding regions (trnL-trnF, trnM-trnV and trnS-trnG) from the chloroplast genome and internal transcribed spacer (ITS) region from the nuclear genome were evaluated among 75 individuals of five species of Rehmannia, and identification efficiency was assessed using PWG-distance and Tree-Building methods. Results indicated that the five Rehmannia species could not be accurately distinguished by single or the combination of chloroplast DNA fragments with low species discrimination rates (0%-20%), but could by the combination of chloroplasts and nuclear DNA regions (trnS-trnG+ITS) with 100% discrimination rates. Thus, trnS-trnG+ITS can be considered as a potential barcode for species identification in Rehmannia. The combination of chloroplasts and nuclear fragments can be widely adopted for the discrimination of plant species when a single DNA barcode fails.

     

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