Effects of Intracellular Distribution of Sugar and Acid at Different Developmental Stages on Sweetness and Sourness of Peach Fruit
JIANG Feng-Chao1,2, WANG Yu-Zhu1,2, SUN Hao-Yuan2, YANG Li2, ZHANG Jun-Huan2, MA Lü-Yi1
1. Academy of Forestry, Beijing Forestry University, Beijing 100083;
2. Institute of Forestry and Pomoloy, Beijing Academy of Agriculture and Forestry Science, Beijing 100093
Abstract：To understand the distribution and changes of sugars and acids in peach fruit during the growing stages, their distribution and concentrations in vacuoles, cytoplasm, and free space in fruits of Prunus persicae‘Hakuho’ at different growing stages were studied by compartmental analysis, and their contribution to fruit sweetness and sourness was assessed. Results showed that the concentration of sugars (sucrose, glucose, fructose and sorbitol) and acids (malate, citrate, quinate and shikimate) in vacuoles, cytoplasm and free space of mature fruits was 27.3, 11.6 and 9.0 mg/g, and 2.09, 0.94 and 0.35 mg/g, respectively, and was 0.97, 2.2 and 2.3 mg/g, and 0.25, 0.44 and 0.82 mg/g in immature fruit, respectively. Sweetness and sourness was less affected by the different distribution of sugar and acid in cells in immature fruit than that in mature fruit. There was an obvious concentration gradient between the vacuoles, cytoplasm and free space for sugars and acids, and the differences in the intracellular distribution of sugar and acid may be responsible for the changes of fruit sweetness.
姜凤超, 王玉柱, 孙浩元, 杨丽, 张俊环, 马履一. 桃果实不同发育时期细胞内糖酸分布对甜酸风味的影响[J]. 植物科学学报, 2014, 32(3): 265-272.
JIANG Feng-Chao, WANG Yu-Zhu, SUN Hao-Yuan, YANG Li, ZHANG Jun-Huan, MA Lü-Yi. Effects of Intracellular Distribution of Sugar and Acid at Different Developmental Stages on Sweetness and Sourness of Peach Fruit. , 2014, 32(3): 265-272.
[1] Gautier H,Diakou-Verdin V,Bénard C,Reich M,Buret M, Bourgaud F,Poëssel JL,Caris-Veyrat C,Génard M.How does tomato quality (sugar,acid,and nutritional quality) vary with ripening stage,temperature,and irradiance?[J].J Agr Food Chem,2008,56(4):1241-1250.[2] 潘腾飞,李永裕,邱栋梁.果实品质形成的分子机理研究进展[J].亚热带植物科学,2006,35(1):81-84.[3] 牛景,赵剑波,吴本宏,李绍华,刘国杰,姜全.不同来源桃种质果实糖酸组分含量特点的研究[J].园艺学报,2006,33(1):6-11.[4] Crisosto CH,Crisosto G,Bowerman E.Searching for consumer satisfaction:new trends in the California peach industry[C]//Marra F,Sottile F,eds.Proceedings of the First Mediterranean Peach Symposium.Agrigento,Italy,2003:113-118.[5] Crisosto CH,Crisosto GM.Relationship between ripe soluble solids concentration (RSSC) and consumer acceptance of high and low acid melting flesh peach and nectarine (Prunus persica (L.) Batsch) cultivars[J].Postharvest Biol Tec,2005,38(3):239-246.[6] Crisosto CH,Crisosto GM,Metheney P.Consumer acceptance of ‘Brooks’ and /cherries is mainly dependent on fruit SSC and visual skin color[J].Postharvest Biol Tec,2003,28(1):159-167.[7] 吴本宏,李绍华,Quilot B,Genard M,Kervella J.桃果皮毛、果肉颜色对果实糖与酸含量的影响及相关性研究[J].中国农业科学,2003,36(12):1540-1544.[8] Cliff MA,Stanich K,Hampson C.Consumer research explores acceptability of a new Canadian apple-Salish[J].Can J Plant Sci,2014,94(1):99-108.[9] Yamaki S.Isolation of vacuoles from immature apple fruit flesh and compartmentation of sugars,organic acids,phenolic compounds and amino acids[J].Plant Cell Physiol,1984,25(1):151-166.[10] Yamada H,Kaga Y,Amano S.Cellular compartmentation and membrane permeability to sugars in relation to early or high temperature-induced watercore in apples[J].Sci Hortic,2006,108(1):29-34.[11] Yamaki S,Ino M.Alteration of cellular compartmentation and membrane permeability to sugars in immature and mature apple fruit[J].J Am Soc Hortic Sci,1992,117(6):951-954.[12] Yamaki S, Ino M, Ozaki S.Tsuchimoto Y,Ofosu-Anim J.Cellular compartmentation and transport into tonoplast vesicles of sugars with ripening of pear fruit[J].Physiological Basis of Postharvest Technologies,1992,343:12-17.[13] Ofosu-Anim J, Yamaki S.Sugar content,compartmentation,and efflux in strawberry tissue[J].J Am Soc Hortic Sci,1994,119(5):1024-1028.[14] Ofosu-Anim J, Yamaki S.Sugar content and compartmentation in melon (Cucumis melo) fruit and the restriction of sugar efflux from flesh tissue by ABA[J].J Jpn Soc Hortic Sci,1994,63:685-692.[15] Macklon AES.Cortical cell fluxes and transport to the stele in excised root segments of Allium cepa L.[J].Planta,1975,122(2):109-130.[16] Seymour GB,stergaard L,Chapman NH,Knapp S,Martin C.Fruit development and ripening[J].Annu Rev Plant Biol,2013,64:219-241.[17] Inzé D,De Veylder L.Cell cycle regulation in plant development[J].Annu Rev Genet,2006,40:77-105.[18] Patrick JW,Botha FC,Birch RG.Metabolic engineering of sugars and simple sugar derivatives in plants[J].Plant Biotechnol J,2013,11(2):142-156.[19] Wada H,Matthews MA,Shackel KA.Seasonal pattern of apoplastic solute accumulation and loss of cell turgor during ripening of Vitis vinifera fruit under field conditions[J].J Exp Bot,2009,60 (6):1773-1781.[20] Marsh KB,Friel EN,Gunson A,Lund C,MacRae E.Perception of flavour in standardised fruit pulps with additions of acids or sugars[J].Food Qual Prefer,2006,17(5):376-386.[21] Lindemann B.Receptors and transduction in taste[J].Nature,2001,413(6852):219-225.[22] Snelgar WP,Hopkirk G,Seelye RJ,Martin PJ,Manson PJ.Relationship between taste perception,density and soluble solids concentration in kiwifruit (Actinidia deliciosa)[J].New Zealand Journal of Crop and Horticultural Science,2009,37:303-317.
2014, Vol. 32 
