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孙晓春, 闫桂琴. 牛奶子树不同部位多糖抗氧化活性比较研究[J]. 植物科学学报, 2011, 29(6): 734-737.
引用本文: 孙晓春, 闫桂琴. 牛奶子树不同部位多糖抗氧化活性比较研究[J]. 植物科学学报, 2011, 29(6): 734-737.
SUN Xiao-Chun, YAN Gui-Qin. Comparisons of Antioxidant Activity of the Polysaccharide from Different Parts of Elaeagnus umbellata Thunb.[J]. Plant Science Journal, 2011, 29(6): 734-737.
Citation: SUN Xiao-Chun, YAN Gui-Qin. Comparisons of Antioxidant Activity of the Polysaccharide from Different Parts of Elaeagnus umbellata Thunb.[J]. Plant Science Journal, 2011, 29(6): 734-737.

牛奶子树不同部位多糖抗氧化活性比较研究

Comparisons of Antioxidant Activity of the Polysaccharide from Different Parts of Elaeagnus umbellata Thunb.

  • 摘要: 从牛奶子(Elaeagnus umbellata Thunb.)的叶片、枝条、种子中提取多糖,并研究其抗氧化活性。采用热水提取、Savage法除蛋白、80%乙醇沉淀得其粗多糖。以Vc为对照,用番红花红光度法测定其对羟基自由基的清除作用;用邻苯三酚自氧化法测定其对超氧阴离子的清除作用;用Fe3+还原力法测定其还原能力。结果表明:3个部位所含多糖对羟基自由基、超氧阴离子自由基都可起到有效的清除或抑制作用,其中对羟基自由基清除活性依次为:种子>叶片>枝条>Vc,对超氧阴离子自由基清除活性依次为:种子>Vc>叶片>枝条,3个部位所含多糖都有还原性,但效果都不如硫脲。

     

    Abstract: Polysaccharide of branches,leaves,and seeds of Elaeagnus umbellata Thunb.were extracted with hot water.Protein was excluded using the savage method,and then concentrated and precipitated with 80% ethanol to obtain the polysaccharide.Antioxidant activity of polysaccharide was evaluated by the efficiency of scavenging the hydroxyl radical and superoxide anion radical and reductive potential,with ascorbic acid used as a positive control.Results showed that the polysaccharide with three parts was effective in removing hydroxyl radical,superoxide free radicals,the scavenging activity on hydroxyl radical from the highest to the lowest was seeds>leaves>branches>Vc,while superoxide removal activity from the highest to the lowest was seeds>Vc>leaves>branches.All of polysaccharides possessed strong reducing power,but the effect was not as Thiourea.

     

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