藏药纤毛婆婆纳的愈伤组织诱导和快速繁殖研究

Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant,Veronica ciliate Fisch.

摘要: 以纤毛婆婆纳(Veronica ciliate Fisch.)无菌苗的顶芽作为外植体,在不同激素和浓度组合的MS培养基上进行愈伤组织诱导和快速繁殖的研究。结果表明,愈伤组织诱导的最佳培养基为:MS+6-BA 0.5 mg/L+NAA1.0 mg/L,诱导率达到95%;顶芽在MS+6-BA 0.5 mg/L+NAA 0.1 mg/L增殖培养基中增殖效果最佳,增殖系数高达5.4;丛生芽在1/2 MS+IBA 0.05 mg/L生根培养基生根效果最好,不仅根的质量好,而且生根率也达到95%;此再生苗的移栽成活率也最高,在适宜条件下可达40%。

Abstract: To induce callus and establish rapid propagation systems for Veronica ciliate Fisch.,terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA.Results showed that the optimum medium for callus inducement was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,with the callus induction rate of 95%.The best medium for multiplication was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,with the multiplication coefficient reaching 5.4.The optimum root medium for multiple shoot clumps was 1/2 MS+IBA 0.05 mg/L,with the rooting rate reaching 95% and the root being healthiest.Under suitable hardening and transplanting conditions,the highest survival rate for the regenerated plantlets reached 40%.