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祝莉莉, 张春晓, 潘玉芳, 陈荣智. 外源糖对水稻Ugp1基因表达调控研究[J]. 植物科学学报, 2011, 1(4): 486-492.
引用本文: 祝莉莉, 张春晓, 潘玉芳, 陈荣智. 外源糖对水稻Ugp1基因表达调控研究[J]. 植物科学学报, 2011, 1(4): 486-492.
ZHU Li-Li, ZHANG Chun-Xiao, PAN Yu-Fang, CHEN Rong-Zhi. Sugar Regulation of Ugp1 Gene Expression in Rice[J]. Plant Science Journal, 2011, 1(4): 486-492.
Citation: ZHU Li-Li, ZHANG Chun-Xiao, PAN Yu-Fang, CHEN Rong-Zhi. Sugar Regulation of Ugp1 Gene Expression in Rice[J]. Plant Science Journal, 2011, 1(4): 486-492.

外源糖对水稻Ugp1基因表达调控研究

Sugar Regulation of Ugp1 Gene Expression in Rice

  • 摘要: 植物尿苷二磷酸葡萄糖焦磷酸化酶(UGPase)是蔗糖合成与降解途径的关键酶。本研究采用水稻叶片离体培养方法,结合Northern杂交技术,研究了外源糖对水稻Ugp1基因表达的影响。研究结果表明,蔗糖、葡萄糖、果糖、光照均能上调水稻Ugp1基因的表达,同时这种上调表达依赖于己糖激酶;果糖能上调水稻成熟叶片中Ugp1基因的表达,但并不影响苗期叶片中Ugp1基因的表达,具组织特异性;葡萄糖和果糖协同作用对Ugp1基因的诱导表达强于蔗糖,这种诱导除依赖于己糖激酶外,还存在其它未知的调控途径。水稻中存在UGPase的多种异构体,蔗糖及光照可诱导水稻Ugp1基因的上调表达,但对水稻UGPase的多种异构体形式并无影响。研究结果将有助于深入了解水稻Ugp1基因与糖信号途径互作调控网络。

     

    Abstract: UDP-glucose pyrophosphorylases(UGPases,EC 2.7.7.9) are key enzymes in plant carbohydrate metabolism and cell wall biosynthesis,catalyzing the reversible production of glucose-1-phosphate and UTP from UDP-glucose and pyrophosphate.The rice(Oryza sativa L.) genome contains two homologous UGPase genes,Ugp1 and Ugp2.The Ugp1 gene is globally expressed throughout the plant and is critical for plant growth and pollen development.In the present study,possible effects of sugars on Ugp1 expression were tested with excised rice leaves.The Ugp1 was up-regulated by feeding sucrose,glucose and fructose to excised rice leaves.The sucrose effect on Ugp1 was mimicked by exposure of dark-adapted leaves to light.The sugar signaling pathway for Ugp1 expression was dependent on hexokinase,as N-acetyl-glucosamine(NAG,a specific HXK inhibitor) was effective in inhibiting sugar-mediated up-regulation of Ugp1.However,NAG could only partially inhibit Ugp1 expression induced by glucose together with fructose,suggesting an unknown signaling pathway was also critical for regulation of Ugp1 expression.Using two-dimensional gel electrophoresis in combination with western blot analysis,we also demonstrated that sucrose-mediated up-regulation of Ugp1 had no effect on multiple rice UGPase isoforms.

     

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