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杨文静, 乔麟轶, 李欣, 郭慧娟, 陈芳, 张树伟, 常利芳, 贾举庆, 畅志坚, 张晓军. 小麦抗条锈基因Yr69的连锁标记开发[J]. 植物科学学报, 2022, 40(2): 197-204. DOI: 10.11913/PSJ.2095-0837.2022.20197
引用本文: 杨文静, 乔麟轶, 李欣, 郭慧娟, 陈芳, 张树伟, 常利芳, 贾举庆, 畅志坚, 张晓军. 小麦抗条锈基因Yr69的连锁标记开发[J]. 植物科学学报, 2022, 40(2): 197-204. DOI: 10.11913/PSJ.2095-0837.2022.20197
Yang Wen-Jing, Qiao Lin-Yi, Li Xin, Guo Hui-Juan, Chen Fang, Zhang Shu-Wei, Chang Li-Fang, Jia Ju-Qing, Chang Zhi-Jian, Zhang Xiao-Jun. Development of linkage markers for stripe rust resistance gene Yr69 in Triticum aestivum L.[J]. Plant Science Journal, 2022, 40(2): 197-204. DOI: 10.11913/PSJ.2095-0837.2022.20197
Citation: Yang Wen-Jing, Qiao Lin-Yi, Li Xin, Guo Hui-Juan, Chen Fang, Zhang Shu-Wei, Chang Li-Fang, Jia Ju-Qing, Chang Zhi-Jian, Zhang Xiao-Jun. Development of linkage markers for stripe rust resistance gene Yr69 in Triticum aestivum L.[J]. Plant Science Journal, 2022, 40(2): 197-204. DOI: 10.11913/PSJ.2095-0837.2022.20197

小麦抗条锈基因Yr69的连锁标记开发

Development of linkage markers for stripe rust resistance gene Yr69 in Triticum aestivum L.

  • 摘要: 抗条锈病基因Yr69对我国小麦条锈菌(Puccinia striiformis f.sp.tritici)小种具有广谱抗性,在小麦抗条锈病育种中具有重要价值。为提高分子标记辅助选择育种的效率,加快Yr69在小麦抗病育种中的应用,本研究利用条锈菌小种CYR34对包含340个小麦家系的‘Taichung29/CH7086’F9代RIL (Recombinant inbred line)群体进行接种鉴定,并利用BSA-SNP (Bulked segregant analysis-single nucleotide polymorphism)技术对其抗条锈病基因进行了重新定位。抗病鉴定结果显示,RIL群体中抗感病家系的数量呈双峰分布,‘CH7086’的条锈病抗性受一个主效位点控制。BSA-SNP基因分型结果表明,多态性SNP主要集中于小麦2AS染色体末端0 ~ 30 Mb的染色体区段。在该基因组区段开发了208个SSR分子标记,利用抗感病小群体从中筛选到14个与Yr69连锁的分子标记。利用14个标记对340个RIL家系进行PCR扩增和分子作图,将Yr69定位于2AS111和2AS171之间约7.76 Mb的染色体区段,两侧连锁标记2AS111、2AS171与Yr69的连锁距离均为0.4 cM,并获得2个与Yr69共分离的分子标记2AS117和2AS127,可用于Yr69的分子标记辅助选择。

     

    Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating wheat diseases in China. The Yr69 gene confers resistance to almost all Chinese P. striiformis stripe rust races, and thus has important value in the breeding of Triticum aestivum L. stripe rust resistance. To improve the efficiency of molecular marker-assisted selection and accelerate the application of Yr69 in wheat resistance breeding, this study used stripe rust race CYR34 to inoculate and identify the F9 recombinant inbred line (RIL) population of ‘Taichung29/CH7086’ containing 340 families. Bulked segregant analysis-single nucleotide polymorphism (BSA-SNP) was used to remap the stripe rust resistance gene in ‘CH7086’. Based on the disease resistance identification results, the number of resistant families and susceptible families in the RIL population exhibited a bimodal distribution, suggesting that the resistance of ‘CH7086’ to stripe rust was controlled by a major locus. The BSA-SNP results showed that the polymorphic SNPs were mainly concentrated in a 0-30 Mb section at the end of the wheat 2AS chromosome. In this segment, 208 simple sequence repeat (SSR) molecular markers were identified. Among these, 14 molecular markers linked to Yr69 were screened using a small population of resistant plants and susceptible plants. The 14 linked markers were used for linkage mapping with 340 F9 RIL population, with two markers found to be co-segregated with the Yr69 gene. Based on the Chinese spring wheat genome IWGSC Ref Seq v1.0, Yr69 was mapped to a 7.76 Mb interval between the two molecular markers 2AS111 and 2AS171, and their linkage distances with Yr69 were both 0.4 cM. Thus, two molecular markers (2AS117 and 2AS127) co-segregated with Yr69 were obtained, which not only can be used for molecular marker-assisted selection of Yr69, but also have important significance for fine mapping and cloning of the gene.

     

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