Abstract:
The
matK gene plays an important role as the only group Ⅱ intros maturase in the chloroplast. We carried out bioinformatics analysis of the
matK gene from five species of
Botrychium. We then discussed the differences in
matK gene sequences and MATK protein amino acid sequences in phylogenetic development of
Botrychium. Results showed that the full length of the
matK gene of the five species ranged from 1500-1503 bp, with 153 variation sites. The encoding products were all unstable hydrophilic proteins without a transmembrane structure, and the secondary structures were mainly alpha helices and random coils. Phylogenetic results showed that the phylogenetic tree constructed from the
matK gene sequence was suitable for the identification of species of
Botrychium. This study enriches current molecular information on
matK in
Botrychium and establishes the application value of
matK in the identification of
Botrychium.
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