中国辣椒热胁迫转录因子的全基因组鉴定及热胁迫响应的初步分析

Genome-wide identification of heat stress transcription factors and preliminary analysis of heat stress responses in Capsicum chinense Jacq.

摘要: 采用生物信息学方法，从中国辣椒（Capsicum chinense Jacq.）全基因组序列中鉴定得到28个热胁迫转录因子（HSF）候选基因，并对这些候选基因的染色体分布、基因结构及编码蛋白的3D结构特征进行了分析。结果显示：28个候选基因的编码蛋白长度为128 ～ 526 aa；系统发育分析结果表明，HSF可分为A、B、C 3个亚家族。进一步对热胁迫处理后的中国辣椒种质进行转录组分析，共检测到27个HSF转录本，与对照组相比，实验组中有25个基因对热胁迫有不同程度的响应。

Abstract: Based on bioinformatics, 28 HSF candidate genes were identified in the whole genome sequence of Capsicum chinense Jacq. The chromosome distribution, gene structure, and 3D characteristics of these candidate genes were analyzed. Results showed that the protein length of the 28 candidate genes ranged from 128 to 526 aa. Phylogenetic analysis showed that HSF could be divided into A, B, and C subfamilies. In total, 27 HSF transcripts were detected by RNA-seq. Compared with the control group, 25 genes in the experimental group exhibited different responses under heat stress.